Assessment of Safe Drinking Water Handling Practices in Households of Northern India: A Cross-Sectional Study.

Background Waterborne diseases are the most common form of infectious disease, spreading from contaminated water, especially in a developed country. These diseases are a major concern for the environment and public health. The living conditions in developing countries like India affect the water-handling practices, which make the population vulnerable to waterborne diseases. The inability to access safe drinking water also adds to this. Water safety for a community relies on water collection, treatment, storage, and handling in the household setting. Therefore, the burden of waterborne disease can be reduced by treating point-of-use drinking water, including improving handling and transport. Objectives The aim was to assess the safe drinking water handling practices in households. The objectives were to assess the safe drinking water-handling practices, namely, treatment, storage, lid status of the storage vessel, and water drawing technique, and to estimate the sources of safe drinking water. Methods This cross-sectional study was conducted in the Etawah district on a total of 312 eldest female family members actively working in the kitchen. Descriptive analysis and Chi-Square test were applied to the collected data and a p-value <0.05 at 95% confidence interval (CI) was taken as statistically significant. Results Overall, 135 (85.9%) households in urban areas relied on public supply. However, in rural areas mostly 130 (83%) households depended on private supply. In water-handling practices, 276 (88.4%) used some method to purify drinking water, a total of 209 (67%) households kept the lid of the storage container covered, and 249 (79.8%) households drew water either by pouring or scooping with a long handle. Conclusion The study concluded that both private and public sources were used for drinking water. Regarding water-handling practices, most households drank purified water, kept their containers covered, and drew water either by scooping or pouring from storage containers. Those who drank purified water mostly belonged to nuclear families and had private sources of drinking water.

Biogenic element driving mechanism in the occurrence of Chinese coastal eco-environmental disasters and regulation for ecological environment health.

In recent decades, China's coastal ecological environment has undergone significant changes under multiple pressures such as climate change and high intensity of human activities. The occurrence and scale of harmful algal blooms represented by the red tide and the green tide are increasing rapidly. Moreover, environmental problems such as hypoxia and acidification in seawater have become increasingly prominent. It is urgent to find out the occurrence mechanism and prevention measures of marine eco-environmental disasters. Therefore, we explained the connotation of the concept of "marine eco-environmental disaster" for the first time, and systematically interpreted the biogenic element driving mechanisms in the occurrence of eco-environmental disasters in China nearshore area in the aspects of exogenous input of nutrients, the mineralization and decomposition of marine organic matters and nutrient regeneration, as well as the abnormal nutrient structure in offshore waters. We pointed out that the drastic increases of terrigenous nutrient discharge caused by enhancing human activities, together with the complex biogeochemical cycle process after biogenic elements entering the sea, led to the frequent occurrence of coastal eco-environmental disasters. On this basis, combined with the latest research progress in this field, we put forward the regulatory schemes for coastal ecological environment health based on the controlling of seawater biogenic element. That is, based on land-sea integration strategy, the reduction of land-source pollutants, especially inorganic nitrogen from land, coastal zone and into the sea, and the normalization of nutrient structure in coastal seawater through artificial regulation technology, are the key points to reduce and control the occurrence of disasters and improve the quality of the coastal ecosystems.

Integrated Assessment of Water Environmental Health Risk through Drinking Water Ingestion in Lhasa River Basin: Quantitative Analysis and Environmental Significance.

An integrated assessment of water environmental health risk through drinking water ingestion in Lhasa River has been implemented. The health risks caused by different pollutants in different age groups (children, juvenile and adult groups) are in the order of 10-8 ~ 10-7, 10-7 ~ 10-5 and 10-13 ~ 10-8 a-1, respectively. The total health risks for all age groups are lower than the International Commission on Radiation Protection recommended value and the U.S. Environmental Protection Agency recommended value at all points except LS4, LS12 and LS13. The total health risk levels in different age groups at most points are class III or II, which means that there are low or negligible negative effect in these points and age groups. It is more important to monitor the arsenic concentration. The water environment quality protecting in Lhasa River Basin must be consistent with the clear water and blue sky protecting in Tibet Autonomous Region and the national ecological security barrier construction on the Tibetan Plateau.

Using environmental health dialogue in a Diné-centered approach for individualized results reporting in an environmental exposure study following the Gold King Mine Spill.

BACKGROUND: On August 5, 2015, the Gold King Mine Spill (GKMS) resulted in 3 million gallons of acid mine drainage spilling into the San Juan River impacting the Diné Bikeyah (traditional homelands of the Navajo people). The Gold King Mine Spill Diné Exposure Project was formed to understand the impacts of the GKMS on the Diné (Navajo). Reporting individualized household results in an exposure study is becoming more common; however, materials are often developed with limited community input with knowledge flowing in one direction - from researcher to participant. In this study we examined the development, dissemination, and evaluation of individualized results materials. METHODS: In August 2016, Navajo Nation Community Health Representatives (Navajo CHRs) sampled household water, dust, and soil, and resident blood and urine for lead and arsenic, respectively. From May-July 2017, iterative dialogue with a wide range of community partners and a community focus groups guided the development of a culturally-based dissemination process. In August 2017, Navajo CHRs reported individualized results and they surveyed the participants on the report-back process at that time. RESULTS: All of the 63 Diné adults (100%) who participated in the exposure study received their results by a CHR in person and 42 (67%) completed an evaluation. Most of those participants (83%) were satisfied with the result packets. Respondents ranked the individual and overall household results as the most important information they received (69% and 57%, respectively), while information on metals exposures and their health effects were the least helpful. CONCLUSIONS: Our project illustrates how a model of environmental health dialogue, defined by iterative, multidirectional communication among Indigenous community members, trusted Indigenous leaders, Indigenous researchers, non-Indigenous researchers, can improve reporting individualized study results. Findings can inform future research to encourage multi-directional environmental health dialogue to craft more culturally responsive and effective dissemination and communication materials.

Hazardous impacts of glyphosate on human and environment health: Occurrence and detection in food.

With the ever-increasing human population, farming lands are decreasing every year, therefore, for effective crop management; agricultural scientists are continually developing new strategies. However, small plants and herbs always impart a much loss in the yields of the crop and farmers are using tons of herbicides to eradicate that problem. Across the world, several herbicides are available in the market for effective crop management, however, scientists observed various environmental and health effects of the herbicides. Over the past 40 years, the herbicide glyphosate has been used extensively with the assumption of negligible effects on the environment and human health. However, in recent years, concerns have increased globally about the potential direct and indirect effects on human health due to the excessive use of glyphosate. As well, the toxicity on ecosystems and the possible effects on all living creatures have long been at the center of a complex discrepancy about the authorization for its use. The World Health Organization also further classified glyphosate as a carcinogenic toxic component and it was banned in 2017 due to numerous life-threatening side effects on human health. In the present era, the residues of banned glyphosate are more prevalent in agricultural and environmental samples which are directly affecting human health. Various reports revealed the detailed extraction process of glyphosate from different categories of the food matrix. Therefore, in the present review, to reveal the importance of glyphosate monitoring in the food matrix, we discussed the environmental and health effects of glyphosate with acute toxicity levels. Also, the effect of glyphosate on aquatic life is discussed in detail and various detection methods such as fluorescence, chromatography, and colorimetric techniques from different food samples with a limit of detection values are revealed. Overall, this review will give an in-depth insight into the various toxicological aspects and detection of glyphosate from food matrix using various advanced analytical techniques.

Public health implications of endocrine disrupting chemicals in drinking water and aquatic food resources in Nigeria: A state-of-the-science review.

This state-of-the-science review is aimed at identifying the sources, occurrence, and concentrations of EDCs, including potential public health risks associated with drinking water and aquatic food resources from Nigerian inland waters. A total of 6024 articles from scientific databases (PubMed, Scopus, Web of science, ScienceDirect, Google Scholar, and African Journals Online) were identified, out of which, 103 eligible articles were selected for this study. Eleven (11) classes of EDCs (OCPs, PCBs, PBDEs, PAHs, BPA, OTs, PEs, PCs, PPCPs, sterols and n-alkanes) were identified from drinking waters, river sediments and aquatic food species from Nigerian rivers, showing that OCPs were the most studied and reported EDCs. Analytical methods used were HPLC, LC-MS/MS, GC-FID, GC-ECD and GC-MS with all EDCs identified to originate from anthropogenic sources. Carcinogenic, mutagenic, and teratogenic effects were the highest (54.4 %) toxicological effects identified, while reproductive/endocrine disruptive effects (15.2 %) and obesogenic effects (4.3 %) were the least identified toxicological effects. The targeted hazard quotient (THQ) and cancer risk (CR) were generally highest in children, compared to the adult populations, indicating age-specific toxicity. PEs produced the highest THQ (330.3) and CR (1.2) for all the EDCs in drinking water for the children population, suggesting enhanced vulnerability of this population group, compared to the adult population. Due to associated public health, wildlife and environmental risk of EDCs and their increasing concentrations in drinking water and food fish species from Nigerian inland waters, there is an urgent need for focused and strategic interventions, sensitization and policy formulation/implementation towards public health and aquatic food safety in Nigeria.

Utility of cockroach as a model organism in the assessment of toxicological impacts of environmental pollutants.

Environmental pollution is a global concern because of its associated risks to human health and ecosystem. The bio-monitoring of environmental health has attracted much attention in recent years and efforts to minimize environmental contamination as well as to delineate toxicological mechanisms related to toxic exposure are essential to improve the health conditions of both humans and animals. This review aims to substantiate the need and advantages in utilizing cockroaches as a complementary, non-mammalian model to further understand the noxious impact of environmental contaminants on humans and animals. We discuss recent advances in neurotoxicology, immunotoxicology, reproductive and developmental toxicology, environmental forensic entomotoxicology, and environmental toxicology that corroborate the utility of the cockroach (Periplaneta americana, Blaptica dubia, Blattella germanica and Nauphoeta cinerea) in addressing toxicological mechanisms as well as a sensor of environmental pollution. Indeed, recent improvements in behavioural assessment and the detection of potential biomarkers allow for the recognition of phenotypic alterations in cockroaches following exposure to toxic chemicals namely saxitoxin, methylmercury, polychlorinated biphenyls, electromagnetic fields, pharmaceuticals, polycyclic aromatic hydrocarbon, chemical warfare agents and nanoparticles. The review provides a state-of-the-art update on the current utility of cockroach models in various aspects of toxicology as well as discusses the potential limitations and future perspectives.

Parasites of Selected Freshwater Snails in the Eastern Murray Darling Basin, Australia.

Aquatic snails serve an important role in the ecosystem. They also play an essential role in the life cycle of many parasites as hosts and may pose risks to animal and human health. In Australia, the role of snails in the transmission of parasites of livestock is well studied. However, despite the country's unique biodiversity and wildlife, little is known about the role of snails in the transmission and survival of parasites in other ecosystems, including aquatic and aquaculture systems. This study aimed to determine the occurrence of parasites in freshwater snails in the eastern Murray Darling Basin. A total of 275 snails were collected from various localities, including aquaculture fishery ponds and natural creeks during the summer and autumn months in the southern hemisphere. Three different species of freshwater snails, all common to the area, were found, including Bullastra lessoni (n = 11), Isidorella hainesii (n = 157), and Haitia acuta (n = 107), of which 9.1%, 1.3%, and 4.7%, respectively, were found to be harboring various developmental stages of Trematoda. No other parasite was found in the examined snails. Parasites were identified as Choanocotyle hobbsi, Plagiorchis sp. and Petasiger sp. based on the sequences of their ITS2, 18S, and 28S ribosomal DNA region. Herein, we report a native parasite Choanocotyle hobbsi in an introduced snail, Haitia acuta, from both natural and aquaculture ponds. As there are no genetic sequences for adult specimens of Petasiger spp. and Plagiorchis spp. collected in Australia for comparison, whether the specimens collected in this study are the larval stage of one of the previously described species or are a new, undescribed species cannot yet be determined. Our results also suggest snails collected from aquaculture ponds may be infected with considerably more parasites.

Setting the European environment and health research agenda -under-researched areas and solution-oriented research.

BACKGROUND: The aim of the EU-funded HERA (health and environment research agenda) project is to set priorities for the future European research agenda in the environment, climate and health nexus. We report results from a European researcher's perspective and identify research areas that have been inadequately investigated to date. METHODS: An online survey was completed by European researchers to assess, evaluate and visualise research gaps. These research gaps were identified for 21 predefined areas within 3 main categories: i) classical environment and health paradigm; ii) problem or sector-based research areas and approaches and iii) holistic research areas and concepts. All research gaps were then evaluated by expert groups with the pre-defined criteria and systematically summarized. For areas identified within the survey as under-reported, additional input was sought from a range of key selected experts. The EU project database Cordis was utilized to verify that these areas were under-researched. RESULTS: Between May and July 2019, 318 respondents from 38 countries reported 624 research gaps. The main areas for attention identified were: urban environments; chemicals; and climate change, (combined n = 313 gaps). Biodiversity loss and health; transport, mobility, sustainable solutions and health; energy transition and health; waste and the circular economy and health; ethics and philosophy and health were areas that were acknowledged as under-researched (combined n = 27 gaps). These under-researched areas were identified as having certain commonalities, they: i) mostly fell in the category "problem or sector based approaches"; ii) they are essential for developing and implementing solutions; and iii) require trans-disciplinary and cross-sectoral collaboration. CONCLUSIONS: Currently attention is given to topical and highly researched areas in environmental health. In contrast, this paper identifies key topics and approaches that are under-researched, yet, are critical for the implementation of the EU Green Deal, related strategies and action plans, and require further investigation and investment. The findings reveal the imperative to foster solutions-oriented, trans-disciplinary and participatory research and its implementation through changes in research funding and research structures.

Heavy metal-induced selection and proliferation of antibiotic resistance: A review.

Antibiotic resistance is recognized as a global threat to public health. The selection and evolution of antibiotic resistance in clinical pathogens were believed to be majorly driven by the imprudent use of antibiotics. However, concerns regarding the same, through selection pressure by a multitude of other antimicrobial agents, such as heavy metals, are also growing. Heavy metal contamination co-selects antibiotic and metal resistance through numerous mechanisms, such as co-resistance and cross-resistance. Here, we have reviewed the role of heavy metals as antimicrobial resistance driving agents and the underlying concept and mechanisms of co-selection, while also highlighting the scarcity of studies explicitly inspecting the process of co-selection in clinical settings. Prospective strategies to manage heavy metal-induced antibiotic resistance have also been deliberated, underlining the need to find specific inhibitors so that alternate medicinal combinations can be added to the existing therapeutic armamentarium.

Validation of a Sustainable Diet Index among young Malaysian adults

@#Introduction: A sustainable diet which is healthy and environmentally friendly provides the means of climate change mitigation in addition to promoting health of the population. There is an urgent need to have an indicator to measure if one’s diet is sustainable. This paper aimed to validate a newly developed Sustainable Diet Index (SDI) among young Malaysian adults. The SDI was developed based on the dietary guidelines of a sustainable diet. Methods: Five indicators (rice, animal-based food, plant-based food, food waste, and packaging) were included in the SDI. The index was validated via content validity, exploratory factor analysis (EFA), and confirmatory factor analysis (CFA) among young Malaysian adults. The dietary assessment tool used was an Android application named Sustainable Food Record. Results: Content validity showed fair to moderate correlations (0.331 - 0.816) between the indicators in the SDI. EFA produced five final factors with eight indicators in the index as follows: 1) fruits and vegetables; 2) dairy, eggs, and meat; 3) rice, cereals, and grain products; 4) food packaging; and 5) food waste management with strong factor loadings (0.760 – 0.984). All five factors with eight indicators were retained and proceeded with CFA. The fit indices from CFA demonstrated that the model was an absolutely fit. Conclusion: The validated SDI can be used as a tool to measure the sustainability of an individual’s diet in Malaysia, incorporating both health and environment considerations.

FAIRification of nanosafety data to improve applicability of (Q)SAR approaches: A case study on in vitro Comet assay genotoxicity data.

(Quantitative) structure-activity relationship ([Q]SAR) methodologies are widely applied to predict the (eco)toxicological effects of chemicals, and their use is envisaged in different regulatory frameworks for filling data gaps of untested substances. However, their application to the risk assessment of nanomaterials is still limited, also due to the scarcity of large and curated experimental datasets. Despite a great amount of nanosafety data having been produced over the last decade in international collaborative initiatives, their interpretation, integration and reuse has been hampered by several obstacles, such as poorly described (meta)data, non-standard terminology, lack of harmonized reporting formats and criteria. Recently, the FAIR (Findable, Accessible, Interoperable, and Reusable) principles have been established to guide the scientific community in good data management and stewardship. The EU H2020 Gov4Nano project, together with other international projects and initiatives, is addressing the challenge of improving nanosafety data FAIRness, for maximizing their availability, understanding, exchange and ultimately their reuse. These efforts are largely supported by the creation of a common Nanosafety Data Interface, which connects a row of project-specific databases applying the eNanoMapper data model. A wide variety of experimental data relating to characterization and effects of nanomaterials are stored in the database; however, the methods, protocols and parameters driving their generation are not fully mature. This article reports the progress of an ongoing case study in the Gov4nano project on the reuse of in vitro Comet genotoxicity data, focusing on the issues and challenges encountered in their FAIRification through the eNanoMapper data model. The case study is part of an iterative process in which the FAIRification of data supports the understanding of the phenomena underlying their generation and, ultimately, improves their reusability.

Impact of Weather Parameters and Population Density on the COVID-19 Transmission: Evidence from 81 Provinces of Turkey.

Weather factors are effective to transmission of various diseases. Middle East Respiratory Syndrome (MERS), Severe Acute Respiratory Syndrome (SARS), and kinds of influenza can be given as example these diseases. The novel corona virus which is called COVID-19 is the most dangerous problem all around the world in these days. Early studies have revealed that COVID-19 cases are affected by environmental factors. Therefore, the purpose of this paper is to examine the relationship between the number of novel coronavirus cases and several weather parameters in 81 provinces of Turkey. Mean incubation period of COVID-19 is in question. Thus, this paper also aims to provide better understanding of the exact incubation period in Turkey by employing four different timeframe which are on the day (lag 0), 3 days ago (lag 3), 7 days ago (lag 7) and 14 days ago (lag 14). We have considered population density as a control variable. The dataset cover COVID-19 cases, population density, average temperature, humidity, pressure, dew point, wind speed, and sunshine duration for 81 provinces of Turkey. We find that population density has a positive correlation with COVID-19 cases. We also find that in lag 3, all parameters except for sunshine duration are negatively correlated with COVID-19 cases and significant. However, only 3 parameters, temperature, air pressure and dew point are negatively correlated with COVID-19 cases and significant for lag 0, lag 7 and lag 14. In addition, temperature, air pressure and dew point parameters are negative and significant in all timeframes.

The one health landscape in Sub-Saharan African countries.

OBJECTIVES: One Health is transiting from multidisciplinary to transdisciplinary concepts and its viewpoints should move from 'proxy for zoonoses', to include other topics (climate change, nutrition and food safety, policy and planning, welfare and well-being, antimicrobial resistance (AMR), vector-borne diseases, toxicosis and pesticides issues) and thematic fields (social sciences, geography and economics). This work was conducted to map the One Health landscape in Africa. METHODS: An assessment of existing One Health initiatives in Sub-Saharan African (SSA) countries was conducted among selected stakeholders using a multi-method approach. Strengths, weaknesses, opportunities and threats to One Health initiatives were identified, and their influence, interest and impacts were semi-quantitatively evaluated using literature reviews, questionnaire survey and statistical analysis. RESULTS: One Health Networks and identified initiatives were spatiotemporally spread across SSA and identified stakeholders were classified into four quadrants. It was observed that imbalance in stakeholders' representations led to hesitation in buying-in into One Health approach by stakeholders who are outside the main networks like stakeholders from the policy, budgeting, geography and sometimes, the environment sectors. CONCLUSION: Inclusion of theory of change, monitoring and evaluation frameworks, and tools for standardized evaluation of One Health policies are needed for a sustained future of One Health and future engagements should be outputs- and outcomes-driven and not activity-driven. National roadmaps for One Health implementation and institutionalization are necessary, and proofs of concepts in One Health should be validated and scaled-up. Dependence on external funding is unsustainable and must be addressed in the medium to long-term. Necessary policy and legal instruments to support One Health nationally and sub-nationally should be implemented taking cognizance of contemporary issues like urbanization, endemic poverty and other emerging issues. The utilization of current technologies and One Health approach in addressing the ongoing pandemic of COVID-19 and other emerging diseases are desirable. Finally, One Health implementation should be anticipatory and preemptive, and not reactive in containing disease outbreaks, especially those from the animal sources or the environment before the risk of spillover to human.

The occurrence of Anisakis spp. in Australian waters: past, present, and future trends.

As one of the world's megadiverse countries, Australian biodiversity is vital for global biodiversity. Nematodes belonging to the genus Anisakis (family Anisakidae) are an important part of this biodiversity due to their ability to be repeatedly transmitted among their intermediate hosts before reaching the top of the food pyramid. Therefore, they have a significant impact on the community structures of various ecosystems. In addition, globally, they are known to be of medical and veterinary significance. The aim of this article is to provide an update on the current knowledge about these important parasites in Australia. Since 1916, a total of 234 records of Anisakis spp. from various hosts and localities have been found in Australia. It is estimated that the occurrence of Anisakis spp. and their health impacts in at least 84, 98.5, and 95% of Australian marine mammals, fish, and water birds, respectively, have not been documented yet. The results of this study suggest Australia is perhaps home to the most diverse Anisakis fauna. Available information is dominated by reports of these parasites in fish hosts, many of them among edible fish. Given the popularity of seafood in Australia and the occurrence of infectious stages of Anisakis spp. in edible fish, all stakeholders should be made aware of the occurrence, prevalence, and survival of Anisakis spp. in seafood. Also, as more pet owners feed their pets with a variety of fish and seafood products, it is important for veterinarians to be aware of seafood transmitted Anisakis spp. in pet animals. This study also highlights several important knowledge gaps: (i) The detailed life cycle of Anisakis spp. in Australia is not known. Detecting their first intermediate hosts is important for better management of crustacean zooplankton populations in our waters. (ii) Research on Anisakis spp. in Australia has been restricted to limited taxonomical studies and should extend to other aspects of these important parasites. (iii) The capacity to identify parasite taxa to species is especially important for resolving biological diversity around Australia; however, opportunities to formally train in parasite taxonomy are rare and diminishing. There is a need to train researchers with taxonomy skills. (iv) Given the vast range of biodiversity in Australia and the broad host-specificity of Anisakis spp., particularly in the larval stages, the full range of their intermediate hosts remains unknown. (v) The health impacts of the infection of the intermediate/definitive hosts with Anisakis spp. are not fully understood. Thus, one of the important areas for future studies is investigating the pathogenicity of Anisakis spp. in affected animals. This is a crucial yet unknown factor for the conservation of some endangered species in Australia.

Application of Quality by Design to the robust preparation of a liposomal GLA formulation by DELOS-susp method.

Fabry disease is a lysosomal storage disease arising from a deficiency of the enzyme α-galactosidase A (GLA). The enzyme deficiency results in an accumulation of glycolipids, which over time, leads to cardiovascular, cerebrovascular, and renal disease, ultimately leading to death in the fourth or fifth decade of life. Currently, lysosomal storage disorders are treated by enzyme replacement therapy (ERT) through the direct administration of the missing enzyme to the patients. In view of their advantages as drug delivery systems, liposomes are increasingly being researched and utilized in the pharmaceutical, food and cosmetic industries, but one of the main barriers to market is their scalability. Depressurization of an Expanded Liquid Organic Solution into aqueous solution (DELOS-susp) is a compressed fluid-based method that allows the reproducible and scalable production of nanovesicular systems with remarkable physicochemical characteristics, in terms of homogeneity, morphology, and particle size. The objective of this work was to optimize and reach a suitable formulation for in vivo preclinical studies by implementing a Quality by Design (QbD) approach, a methodology recommended by the FDA and the EMA to develop robust drug manufacturing and control methods, to the preparation of α-galactosidase-loaded nanoliposomes (nanoGLA) for the treatment of Fabry disease. Through a risk analysis and a Design of Experiments (DoE), we obtained the Design Space in which GLA concentration and lipid concentration were found as critical parameters for achieving a stable nanoformulation. This Design Space allowed the optimization of the process to produce a nanoformulation suitable for in vivo preclinical testing.

Characterization of Clinostomum sp. (Trematoda: Clinostomidae) infecting cormorants in south-eastern Australia.

Clinostomum Leidy, 1856 (Trematoda: Clinostomidae) is a cosmopolitan, zoonotic genus of fluke that has been poorly studied in an Australian setting. Following previous reports of reservoir fish in Australian fish ponds being heavily infected with Clinostomum metacercaria, the current study was conducted to determine the specific identity of Clinostomum sp. in inland Australia, by examining and characterizing parasites collected from a potential definitive host, cormorants. A total of 33 parasite specimens belonging to the genus Clinostomum were collected from two cormorants (little black cormorants, Phalacrocorax sulcirostris) that were collected from the Narrandera Fisheries Research Centre, New South Wales, at the same locality where metacercaria of Clinostomum sp. have been reported in fish. All specimens in our study were immature adults. Clinostomum specimens with similar morphology have been identified as C. complanatum in the past, based on their morphological characteristics. However, phylogenetic analyses based on the ITS sequence data in the present study suggest they are the same as the Clinostomum sp. previously reported from carp gudgeons (Hypseleotris spp.) from the same farm, and distinct from C. complanatum. The ITS sequences obtained from the specimens in the present study were most similar to those belonging to C. phalacrocoracis (never reported in Australia). Our specimens formed a distinct clade on the phylogenetic tree and their specific identity awaits until fully mature specimens are described in future studies.

The occurrence and abundance of infective stages of zoonotic nematodes in selected edible fish sold in Australian fish markets.

Seafood is nutritious and a healthy source of proteins and its regular consumption is highly recommended by medical professionals and dieticians. Owing to this, the global consumption of seafood per capita has been significantly increasing since the 1960s. Consequently, seafood-borne pathogens, including parasites, have also become more widely known and recognised. In Australia, a vast island country, information about such parasites is extremely limited. This study aimed to determine the prevalence and abundance of zoonotic parasites, including anisakid nematodes, in selected Australian edible fish. Four species of fish, namely tiger flathead, Platycephalus richardsoni (n = 43), blue mackerel, Scomber australasicus (n = 117), snapper, Pagrus auratus (n = 11) and school whiting, Sillago flindersi (n = 90) were purchased from a fish market. Although a range of parasites was found, due to their significance for human health, the focus of this study was on nematodes whose infectious stage was found in these fish. The prevalence of nematodes in these fish species was 86.05%, 64.10%, 45.45% and 56.67%, respectively. Among the parasites found, Anisakis spp., Contracaecum spp. and Hysterothylacium spp. in tiger flathead, blue mackerel and school whiting, might be of zoonotic importance. Our findings suggest there is a need to revise current seafood safety protocols and develop educational campaigns for seafood industries stakeholders.

Spectroscopic insight into the pH-dependent interactions between atmospheric heavy metals (Cu and Zn) and water-soluble organic compounds in PM2.5.

Taking Cu and Zn as examples, the pH-dependent interactions between atmospheric heavy metals (AHMs) and water-soluble organic compounds (WSOCs) in PM2.5 were analyzed by a combination of UV-vis absorption, Fourier transform infrared (FTIR) spectroscopy and excitation-emission matrix (EEM) fluorescence spectroscopy coupled with parallel factor analysis (PARAFAC). We found metal-H ion exchange, complexation and electrostatic adsorption might occur between AHMs and WSOCs, and were generally enhanced with the increase of pH. Furthermore, these interactions were strengthened with the stepwise addition of [Cu2+] (from 0 to 500 µmol·L-1), but had a relatively slight change with the stepwise addition of [Zn2+] (from 0 to 500 µmol·L-1) generally. This indicated that the above interactions depended on the types and the concentrations of AHMs. Carboxyl, hydroxyl, carbonyl and aromatic structures of WSOCs were the major binding sites with AHMs. Humic acid-like substances were the dominant components of WSOCs binding with AHMs. The ratios of the apparent fluorescence quantum yields of the low and the high conjugation fractions of WSOCs (QExL/H) declined by more than 28% as adding [Cu2+], indicating the formers had more strong complexing capacity with AHMs. AHMs might significantly impact the light absorption capacity and the wavelength dependence of WSOCs. The humification index (HIXem) declined more than 15% as adding [Cu2+] at pH 5.6 and 7.5, indicating AHMs might weaken the oxidation capacity of WSOCs. These results implied the interactions between AHMs and WSOCs might play a profound role in atmospheric environment, human health, and global climate change.